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Microscopy

 

Actual object size = Image size/magnification I=AxM

Practical:

  • 1. prepare your slide – add drop of water, specimen, iodine then coverslip
  • 2. clip slide onto stage, select lowest power objective lens, use coarse adjustment knob, adjust focus with fine adjustment knob
  • 3. draw observations (in pencil), write title, magnification, label important features

Electron microscopes have a higher resolution

Iodine is needed to stain the sample to highlight objects in the cell by adding colour